ABSTRACT
@#Objective Toinvestigatetheeffectofhydroxyapatite(HA)ontheosteogenicdifferentiationpotencyof adipose-derivedmesenchymalstemcells(ADSCs).Methods ADSCswereisolatedandpurifiedfromC57BL/6mice.Toxic effectsofhydroxyapatiteonADSCsweredetectedbycellproliferationassay.Alkalinephosphatase(ALP)assaywasusedto detecttheeffectsofdifferentconcentrationsofHAonosteogenicdifferentiationofADSCs.RelativemRNAexpressionlevels ofosteogenicgenes(BGLAP, ALP, COL1A1, OPNandRunx2)weremeasuredbyRT-PCR.Results Thelowconcentration ofHA(≤20mg/L)showedlesseffectonproliferationofADSCs.WiththeincreaseofHAconcentration,thecellproliferation decreased.Theco-cultureof20mg/LHAwithADSCssignificantlyincreasedtheALPactivity,andpromotedtheexpression ofosteoblast-relatedgenes(P＜0.01).Conclusion Hydroxyapatitehavetheabilitytoinducetheosteogenicdifferentiation ofADSCs,whichprovideatheoreticalbasisforcombinatingHAandADSCsintoanewboneengineeringscaffold.